Composite

Part:BBa_K912035:Design

Designed by: Yuan Tao   Group: iGEM12_Northwestern   (2012-10-03)


pH Inducible GFP system (s)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1992
    Illegal NheI site found at 2015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2564
    Illegal AgeI site found at 3308
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1901


Design Notes

none


Source

GFP:registry CP/RBS: registry clc-ec1: PCR from genome Pgad: PCR from vector from CUHK 2011 team

References